Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle
نویسندگان
چکیده
منابع مشابه
Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle
BACKGROUND Electrotransfer of plasmid DNA into skeletal muscle is a promising strategy for the delivery of therapeutic molecules targeting various muscular diseases, cancer and lower-limb ischemia. Internal Ribosome Entry Sites (IRESs) allow co-expression of proteins of interest from a single transcriptional unit. IRESs are RNA elements that have been found in viral RNAs as well as a variety of...
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Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES.
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In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi- or oligocistronic expression vectors to co-express various genes from one mRN...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2007
ISSN: 1472-6750
DOI: 10.1186/1472-6750-7-74